| 质粒名称: | aMHC-Puro Rex-Blast |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 21231 |
| 存储实验室: | Mark Mercola |
|
载体骨架基本信息 |
|
| 载体名称: | SMPU |
| 空载体大小: | 7233 bp |
| 完整质粒大小: | 12703 bp |
| 载体类型: | Lentiviral |
| 筛选标记: | Puromycin ; Blasticidin resistanct marker driven by Rex-1 promoter |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | alpha myosin heavy chain promoter / MHC promoter |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 5470bp |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI(未破坏) |
| 3’克隆位点: | AgeI(未破坏) |
| 5’测序引物 | n/a |
|
文献引用方法 |
|
| 材料和方法部分: | aMHC-Puro Rex-Blast was a gift from Mark Mercola (Addgene plasmid # 21231 ; http://n2t.net/addgene:21231 ; RRID:Addgene_21231) |
| 参考文献部分: | Lentiviral vectors and protocols for creation of stable hESC lines for fluorescent tracking and drug resistance selection of cardiomyocytes. Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot JG, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch AD, Price JH, Conklin BR, Chen HS, Mercola M. PLoS ONE. 2009 . 4(4):e5046. 10.1371/journal.pone.0005046 PubMed 19352491 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS aMHC-Puro Rex-Blast 12703 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS aMHC-Puro Rex-Blast
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12703)
AUTHORS Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot
JG, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R,
Calzolari D, Terskikh A, McCulloch AD, Price JH, Conklin BR, Chen
HS, Mercola M
TITLE Lentiviral vectors and protocols for creation of stable hESC lines
for fluorescent tracking and drug resistance selection of
cardiomyocytes.
JOURNAL PLoS ONE. 2009 . 4(4):e5046.
PUBMED 19352491
REFERENCE 2 (bases 1 to 12703)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..12703
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 50..353
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 354..557
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 508..528
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 571..751
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 798..923
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 974..1089
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
primer_bind 1165..1181
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 1165..1181
/label=pBluescriptSK
/note="For pBluescript vector"
CDS 6642..7241
/codon_start=1
/gene="pac from Streptomyces"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
primer_bind complement(6642..6661)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 7138..7158
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
CDS 7985..8383
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature 8389..8977
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8442..8462)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8860..8871)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
protein_bind 9336..9369
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
LTR 9440..9620
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(9670..9689)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(9903..9922)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 10022..10044
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(10082..10100)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 10168..10272
/gene="bla"
/label=AmpR promoter
CDS 10273..11133
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(10491..10510)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 11304..11892
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 11793..11812
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 12046..12063
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(12133..12153)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 12151..12448
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
promoter 12249..12459
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 12318..12453
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 12380..12399
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 12569..12703
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind 12660..12679
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 gggccccaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
61 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
121 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
181 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
241 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
301 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
361 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
421 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
481 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
541 tctatataag cagagctggt ttagtgaacc gggtctctct ggttagacca gatctgagcc
601 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
661 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
721 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ctgaaagcga
781 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
841 gaggcgaggg gcggcgactg cagagtacgc caaaaatttt gactagcgga ggctagaagg
901 agagagatgg gtgcgagagc gtcagtatta agcgggggaa aataagcccg ggatggcagt
961 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
1021 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
1081 tcaaattttc gggtttatta cagggacagc ggcctatggg aatgaaagac cccacctgtc
1141 gagctccacc gcggtggcgg ccgctctaga actagtggat cctgcaaggt cacacaaggg
1201 tctccaccca ccaggtgccc tagtctcaat ttcagtttcc atgccttgtt ctcacaatgc
1261 tggcctcccc agagctaatt tggactttgt ttttatttca aaagggcctg aatgaggagt
1321 agatcttgtg ctacccagct ctaagggtgc ccgtgaagcc ctcagacctg gagcctttgc
1381 aacagccctt taggtggaag cagaataaag caattttcct taaagccaaa atcctgcctc
1441 tagactcttc ttctctgacc tcggtccctg ggctctaggg tggggaggtg gggcttggaa
1501 gaagaaggtg gggaagtggc aaaagccgat ccctagggcc ctgtgaagtt cggagccttc
1561 cctgtacagc actggctcat agatcctcct ccagccaaac atagcaagaa gtgatacctc
1621 ctttgtgact tccccaggcc cagtacctgt caggttgaaa caggatttag agaagcctct
1681 gaactcacct gaactctgaa gctcatccac caagcaagca cctaggtgcc actgctagtt
1741 agtatcctac gctgataata tgcagagctg ggccacagaa gtcctggggt gtaggaactg
1801 accagtgact tttcagtcgg caaaggtatg accccctcag cagatgtagt aatgtcccct
1861 tagatcccat cccaggcagg tctctaagag gacatgggat gagagatgta gtcatgtggc
1921 attccaaaca cagctatcca cagtgtccct tgccccttcc acttagccag gaggacagta
1981 accttagcct atctttcttc ctccccatcc tcccaggaca caccccctgg tctgcagtat
2041 tcatttcttc cttcacgtcc cctctgtgac ttccatttgc aaggcttttg acctctgcag
2101 ctgctggaag atagagtttg gccctaggtg tggcaagcca tctcaagaga aagcagacaa
2161 cagggggacc agattttgga aggatcagga actaaatcac tggcgggcct gggggtagaa
2221 aaaagagtga gtgagtccgc tccagctaag ccaagctagt ccccgagata ctctgccaca
2281 gctgggctgc tcggggtagc tttaggaatg tgggtctgaa agacaatggg attggaagac
2341 atctctttga gtctcccctc aaccccacct acagacacac tcgtgtgtgg ccagactcct
2401 gttcaacagc cctctgtgtt ctgaccactg agctaggcaa ccagagcatg ggccctgtgc
2461 tgaggatgaa gagttggtta ccaatagcaa aaacagcagg ggagggagaa cagagaacga
2521 aataaggaag gaagaaggaa aggccagtca atcagatgca gtcagaagag atgggaagcc
2581 aacacacagc ttgagcagag gaaacagaaa agggagagat tctgggcata aggaggccac
2641 agaaagaaga gcccaggccc cccaagtctc ctctttatac cctcatcccg tctcccaatt
2701 aagcccactc ttcttcctag atcagacctg agctgcagcg aagagacccg tagggaggat
2761 cacactggat gaaggagatg tgtggagaag tccagggcaa cctaagagcc agagcctaaa
2821 agagcaagag ataaaggtgc ttcaaaggtg gccaggctgt gcacacagag ggtcgaggac
2881 tggtggtaga gcctcaagat aaggatgatg ctcagaatgg gcgggggggg ggattctggg
2941 ggggggagag agaaggtgag aaggagcctg gaacagagaa tctggaagcg ctggaaacga
3001 taccataaag ggaagaaccc aggctacctt tagatgtaaa tcatgaaaga cagggagaag
3061 ggaagctgga gagagtagaa ggaccccggg gcaagacatg gaagcaagga caagccaggt
3121 tgagcgctcc gtgaaatcag cctgctgaag gcagagccct ggtatgagca ccagaacagc
3181 agaggctagg gttaatgtcg agacagggaa cagaaggtag acacaggaac agacagagac
3241 gggggagcca ggtaacaaag gaatggtcct tctcacctgt ggccagagcg tccatctgtg
3301 tccacatact ctagaatgtt catcagactg cagggctggc ttgggaggca gctggaaaga
3361 gtatgtgaga gccaggggag acaagggggc ctaggaaagg aagaagaggg caaaccaggc
3421 cacacaagag ggcagagccc agaactgagt taactccttc cttgttgcat cttccatagg
3481 aggcagtggg aactctgtga ccaccatccc ccatgagccc ccactaccca taccaagttt
3541 ggcctgagtg gcattctagg ttccctgagg acagagcctg gcctttgtct cttggacctg
3601 acccaagctg acccaatgtt ctcagtacct tatcatgccc tcaagagctt gagaaccagg
3661 cagtgacata ttaggccatg ggctaaccct ggagcttgca cacaggagcc tcaagtgacc
3721 tccagggaca cagctgcaga caggtggcct ttatccccaa agagcaacca tttggcatag
3781 gtggctgcaa atgggaatgc aaggttgaat caggtccctt caagaatact gcatgcaaga
3841 cctaagaccc ctggagagag gggtatgctc ctgcccccac ccaccataag gggagtgaac
3901 tatcctaggg ggctggcgac cttggggaga caccacatta ctgagagtgc tgagcccaga
3961 aaaactgacc gccctgtgtc ctgcccacct ccacactcta gagctatatt gagaggtgac
4021 agtagatagg gtgggagctg gtagcaggga gagtgttcct gggtgtgagg gtgtagggga
4081 aagccagagc aggggagtct ggctttgtct cctgaacaca atgtctactt agttataaca
4141 ggcatgacct gctaaagacc caacatctac gacctctgaa aagacagcag ccctggagga
4201 caggggttgt ctctgagcct tgggtgcttg atggtgccac aaaggagggc atgagtgtga
4261 gtataaggcc ccaggagcgt tagagaaggg cacttgggaa ggggtcagtc tgcagagccc
4321 ctatccatgg aatctggagc ctggggccaa ctggtgtaaa tctctgggcc tgccaggcat
4381 tcaaagcagc acctgcatcc tctggcagcc tggggaggcg gaagggagca accccccact
4441 tatacccttt ctccctcagc cccaggatta acacctctgg ccttccccct tcccacctcc
4501 catcaggagt ggagggttgc agagggaggg taaaaaccta catgtccaaa catcatggtg
4561 cacgatatat ggatcagtat gtgtagaggc aagaaaggaa atctgcaggc ttaactgggt
4621 taatgtgtaa agtctgtgtg catgtgtgtg tgtctgactg aaaacgggca tggctgtgca
4681 gctgttcagt tctgtgcgtg aggttaccag actgcaggtt tgtgtgtaaa ttgcccaagg
4741 caaagtgggt gaatcccttc catggtttaa agagattgga tgatggcctg catctcaagg
4801 accatggaaa atagaatgga cactctatat gtgtctctaa gctaaggtag caaggtcttt
4861 ggaggacacc tgtctagaga tgtgggcaac agagactaca gacagtatct gtacagagta
4921 aggagagaga ggagggggtg tagaattctc ttactatcaa agggaaactg agtcgtgcac
4981 ctgcaaagtg gatgctctcc ctagacatca tgactttgtc tctggggagc cagcactgtg
5041 gaacttcagg tctgagagag taggaggctc ccctcagcct gaagctatgc agatagccag
5101 ggttgaaagg gggaagggag agcctgggat gggagcttgt gtgttggagg caggggacag
5161 atattaagcc tggaagagaa ggtgaccctt acccagttgt tcaactcacc cttcagatta
5221 aaaataactg aggtaagggc ctgggtaggg gaggtggtgt gagacgctcc tgtctctcct
5281 ctatctgccc atcggccctt tggggaggag gaatgtgccc aaggactaaa aaaaggccat
5341 ggagccagag gggcgagggc aacagacctt tcatgggcaa accttggggc cctgctgtcc
5401 tcctgtcacc tccagagcca agggatcaaa ggaggaggag ccaggacagg agggaagtgg
5461 gagggagggt cccagcagag gactccaaat ttaggcagca ggcatatggg atgggatata
5521 aaggggctgg agcactgaga gctgtcagag atttctccaa cccaggtaag agggagtttc
5581 gggtgggggc tcttcaccca caccagacct ctccccacct agaaggaaac tgcctttcct
5641 ggaagtgggg ttcaggccgg tcagagatct gacagggtgg ccttccacca gcctgggaag
5701 ttctcagtgg caggaggttt ccacaagaaa cactggatgc cccttccctt acgctgtctt
5761 ctccatcttc ctcctgggga tgctcctccc cgtcttggtt tatcttggct cttcgtcttc
5821 agcaagattt gccctgtgct gtccactcca tctttctcta ctgtctccgt gccttgcctt
5881 gccttcttgc gtgtccttcc tttccaccca tttctcactt caccttttct ccccttctca
5941 tttgtattca tccttccttc cttccttcct tccttccttc cttccttcct tccttccttt
6001 ctcccttcct tccttccttc cttccttcct tccttccttc cttcctgtgt cagagtgctg
6061 agaatcacac ctggggttcc cacccttatg taaacaatct tccagtgagc cacagcttca
6121 gtgctgctgg gtgctctctt accttcctca ccccctggct tgtcctgttc catcctggtc
6181 aggatctcta gattggtctc ccagcctctg ctactcctct tcctgcctgt tcctctctct
6241 gtccagctgc gccactgtgg tgcctcgttc cagctgtggt ccacattctt caggattctc
6301 tgaaaagtta accaggtgag aatgtttccc ctgtagacag cagatcacga ttctcccgga
6361 agtcaggctt ccagccctct ctttctctgc ccagctgccc ggcactctta gcaaacctca
6421 ggcaccctta ccccacatag acctctgaca gagaagcagg cactttacat ggagtcctgg
6481 tgggagagcc ataggctacg gtgtaaaaga ggcagggaag tggtggtgta ggaaagtcag
6541 gacttcacat agaagcctag cccacaccag aaatgacaga cagatccctc ctatctcccc
6601 cataagagtt tgagtcgact gatcaaaaac cggtcgccac catgaccgag tacaagccca
6661 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
6721 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
6781 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
6841 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
6901 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
6961 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
7021 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
7081 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
7141 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag tgcccgaagg
7201 accgcgcgac ctggtgcatg acccgcaagc ccggtgcctg ataagggcgc gccaccgatt
7261 cctcccgata agtaaattac agaaaagctt caggttaaat gtaagaggat cttaaatatt
7321 gatttggttt ttgtcttaat ggtgcccggc gtatttataa gaaccattaa atttcactag
7381 accgtttgta gtcaggggtg aacgctgcat gacccacgct ctcaaagtga gttgtgttgc
7441 tttgcgggaa tccagcagtc acattaatgc tggaaaaagt tcaggcaact agtgtacttt
7501 gtagcggggt ccgggagagg ctggggtcta gagtggcgat gggacgaaag ggtaaagaaa
7561 gttttcggtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtaggtagg
7621 taggtaggta gaaactgcat cctctgcttg tgtaaatccg gttacatgtg tgtaacagag
7681 gtactgagat gtgactgagt ctcaaggcca ggcgatcggg attcagaaga ggcatttgca
7741 taactgagca agagcctttg ccccaccctt ccacgcggac ccaagacaag cggacagctc
7801 tgggtgggtc accttgaagc caggggcccg cccacatccc cgcccacacc caccttgagc
7861 gcttctcatt gggcattcca gcctacccag ctcggagtta gttactccgt aagtgtggcc
7921 ggaacagagt tcgtccatct aaaaagggag gggaccggcg aacacatccg gtaggagggc
7981 catcatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
8041 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
8101 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
8161 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
8221 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
8281 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
8341 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taactagaaa tcaacctctg
8401 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
8461 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
8521 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
8581 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
8641 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
8701 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
8761 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
8821 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
8881 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
8941 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtcgacagga gctttgttcc
9001 ttgggttctt gggagcagca ggaagcacta tgggctgcac gtcaatgacg ctgacggtac
9061 aggccagaca attattgtct gatatagtgc agcagcagaa caatttgctg agggctattg
9121 aggcgcaaca gcatctgttg caactcacag tctggggcat caaacagctc caggcaagaa
9181 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggtaccttta agaccaatga
9241 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
9301 taattcactc ccaacgaaga caagatatcc tcgagataac ttcgtatagc atacattata
9361 cgaagttatc agtcgagttt atttgtgaaa tttgtgatgc tattgcttta tttgtaagca
9421 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
9481 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
9541 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
9601 agtgtggaaa atctctagca gccaaacgcg tccgcggtgt acccggcgcc acaggtgcgg
9661 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
9721 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
9781 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
9841 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc
9901 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
9961 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
10021 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
10081 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
10141 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
10201 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
10261 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
10321 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
10381 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
10441 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
10501 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
10561 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
10621 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
10681 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
10741 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
10801 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
10861 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
10921 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
10981 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
11041 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
11101 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
11161 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
11221 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
11281 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
11341 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
11401 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
11461 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
11521 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
11581 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
11641 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
11701 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
11761 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
11821 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
11881 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
11941 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
12001 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
12061 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
12121 taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
12181 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
12241 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
12301 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
12361 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
12421 tccagaagta gtgaggaggc ttttttggag gcctaggcta gagatcataa tcagccatac
12481 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
12541 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
12601 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
12661 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atc
//
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